Journal: Journal of Pharmacopuncture

Article Title: Effects of Acupuncture at ST36 and SP6 on Activity Levels and Pain Thresholds Through Interleukin-1, Interleukin-6, Cortisol, and Matrix Metalloproteinase-13 Modulation in an Osteoarthritis Rat Model

doi: 10.3831/KPI.2026.29.3.286

Figure Lengend Snippet: Serum levels of interleukin (IL)-1 (A) and IL-6 (B) in an untreated osteoarthritis rat model, healthy control rats, and three experimental groups of osteoarthritis model rats following acupuncture treatment. Data are presented as mean ± standard deviation. Statistical analysis was performed using one-way analysis of variance followed by Duncan’s multiple range test. Different letters indicate significant differences between groups (p < 0.05). K−, negative control; K+, positive control; K + A, acupuncture to the ST36 + SP6 points; K + B, acupuncture to the ST36 point only; acupuncture to the K + C, SP6 point only.

Article Snippet: These were the Rat IL-1 ELISA Kit (cat. no. E0119Ra) and the Rat IL-6 ELISA Kit (cat. no. E0135Ra), both from BT-LAB (Shanghai, China).

Techniques: Control, Standard Deviation, Analysis, Negative Control, Positive Control

Journal: Journal of Pharmacopuncture

Article Title: Effects of Acupuncture at ST36 and SP6 on Activity Levels and Pain Thresholds Through Interleukin-1, Interleukin-6, Cortisol, and Matrix Metalloproteinase-13 Modulation in an Osteoarthritis Rat Model

doi: 10.3831/KPI.2026.29.3.286

Figure Lengend Snippet: Pearson correlations between inflammatory and hormonal biomarkers and behavioral outcomes in a monosodium iodoacetate-induced osteoarthritis rat model

Article Snippet: These were the Rat IL-1 ELISA Kit (cat. no. E0119Ra) and the Rat IL-6 ELISA Kit (cat. no. E0135Ra), both from BT-LAB (Shanghai, China).

Techniques: Biomarker Discovery, Activity Assay

Journal: Frontiers in Pharmacology

Article Title: Kaempferol in inflammatory bowel disease: regulating intestinal epithelial-fibroblast crosstalk

doi: 10.3389/fphar.2026.1799034

Figure Lengend Snippet: KF alleviated fibrosis and inflammatory cytokines in mice. (A,B) Masson staining was used to detect colonic fibrosis and collagen deposition (magnification: 200×, scale bars: 150 µm) (each group: n = 6). (C–E) ELISA assay for determining the levels of inflammatory cytokines (each group: n = 6). (F–I) The expression levels of tumor necrosis factor α (TNF-α), zonula occludens-1 (ZO-1) and alpha smooth muscle actin (α-SMA) in colon tissues were measured by western blot. Glyceraldehyde -3-phosphate dehydrogenase (GAPDH) was the internal parameter (each group: n = 6). (J) Transepithelial electrical resistance the Ussing chamber experiment (each group: n = 6). *** P < 0.001 vs. Con; ^ P < 0.05, ^^ P < 0.01, ^^^ P < 0.001 vs. Mod.

Article Snippet: According to the instructions of the ELISA kit (E-EL-M0037, E-EL-M0044, E-EL-M3063, E-EL-H0149, E-EL-H6156, E-EL-H0109, E-EL-0162, E-EL-H2172, E-EL-H0084, Elabscience, China), samples were added sequentially, followed by the incubation with biotinylated antibody working solution and plate washing.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Kaempferol in inflammatory bowel disease: regulating intestinal epithelial-fibroblast crosstalk

doi: 10.3389/fphar.2026.1799034

Figure Lengend Snippet: KF regulated HT29 cell inflammation and oxidative stress induced by TNF-α intervention. (A) Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was employed to determine the cytotoxicity of KF against HT29 cells. (B–D) The protein expression levels of ZO-1 and HMOX1 in each group of cells were assessed by western blot. GAPDH was the internal parameter. (E,F) The level of transforming growth factor-β1 (TGF-β1) and HO-1 in the cell culture medium was detected by enzyme-linked immunosorbent assay (ELISA). (G,H) Western blot was used to examine the NRF2 nuclear translocation. GAPDH, LaminB1 was the internal parameter. (I) Flow cytometry-based detection of intracellular reactive oxygen species (ROS) generation. (J) The level of SOD was detected by commercially available kits. (K–M) ELISA assay for determining the levels of inflammatory cytokine (IL-1β, IL-6 and TNF-α). Each experiment was repeated 3 times. ** P < 0.01, *** P < 0.001 vs. Con; + P < 0.05, +++ P < 0.001 vs. TNF-α; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. KF.

Article Snippet: According to the instructions of the ELISA kit (E-EL-M0037, E-EL-M0044, E-EL-M3063, E-EL-H0149, E-EL-H6156, E-EL-H0109, E-EL-0162, E-EL-H2172, E-EL-H0084, Elabscience, China), samples were added sequentially, followed by the incubation with biotinylated antibody working solution and plate washing.

Techniques: Expressing, Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay, Translocation Assay, Flow Cytometry

Journal: Frontiers in Pharmacology

Article Title: Kaempferol in inflammatory bowel disease: regulating intestinal epithelial-fibroblast crosstalk

doi: 10.3389/fphar.2026.1799034

Figure Lengend Snippet: HO-1 inhibitors reverse the effects of KF, and KF can also influence HT29 cells in a co-culture system. (A) The viability of CCD-18Co cells was examined by CCK-8. (B–E) Western blot was used to quantify the levels of activation marker proteins in CCD-18Co cells, including MMP2/9 and α-SMA. GAPDH was the internal parameter. (F) The viability of HT-29 cells was detected by CCK-8. (G–I) ELISA assay for determining the levels of inflammatory cytokine (IL-1β, IL-6 and TNF-α). (J) ELISA was used to measure the level of hepatocyte growth factor (HGF) in the culture medium. (K,L) The protein level of ZO-1 in HT29 was tested by western blot. GAPDH was the internal parameter. Each experiment was repeated 3 times. + P < 0.05, ++ P < 0.01, +++ P < 0.001 vs. TNF-α; Ω P <0.05 vs. TNF-α+KF; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. Con; # P < 0.05, ### P < 0.001 vs. KF.

Article Snippet: According to the instructions of the ELISA kit (E-EL-M0037, E-EL-M0044, E-EL-M3063, E-EL-H0149, E-EL-H6156, E-EL-H0109, E-EL-0162, E-EL-H2172, E-EL-H0084, Elabscience, China), samples were added sequentially, followed by the incubation with biotinylated antibody working solution and plate washing.

Techniques: Co-culture Assay, CCK-8 Assay, Western Blot, Activation Assay, Marker, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Veterinary Science

Article Title: Functional and biochemical inflammatory responses to a 75 ng intra-articular recombinant equine interleukin-1β (reIL-1β) synovitis model

doi: 10.3389/fvets.2026.1874132

Figure Lengend Snippet: Estimated marginal (EM) mean (± SE) concentrations of prostaglandin E 2 (PGE 2 ; pg/mL), nitric oxide (NO; μM), and glycosaminoglycans (GAG; μg/mL), chondroitin sulfate 846 epitope (CS846; ng/mL), equine resolvin D1 (RvD1; pg/mL) in synovial fluid collected from the intercarpal joints of eleven horses at baseline (0 h) and at 6-, and 12-h following intra-articular injection 75 ng recombinant equine IL-1β (reIL-1β).

Article Snippet: Horses received a single intra-articular injection of 75 ng recombinant equine interleukin-1β (reIL-1β; Kingfisher Biotech, Inc., USA) diluted in 500 μL sterile phosphate-buffered saline and administered into the left intercarpal joint.

Techniques: Injection, Recombinant, Biomarker Discovery